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anti obr antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti obr antibody
    Anti Obr Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+obr/Human+Leptin+R+Antibody/us11965028-257-19-22
    Average 93 stars, based on 5 article reviews
    anti obr antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Neuropilin-1-peptide based antibody, highlighting new epitope associated to glycosaminoglycan-modified neuropilin-1 and methods of use thereof
    Article Snippet: To analyze the a-NRP1_LEP antibody effect on the NRP-1/OBR complex formation, the MDA-MB-231 cells were cultured for two days in human serum (H4522, Sigma) and then treated for two additional days with the mouse a-NRP1_LEP antibody of the invention or its mIgG2b isotype control at 10, 20 or 40 μg/ml, or with the antibody of the prior art a-NRP1_VE and its hIgG1 isotype control at 20 μg/ml. .. After washes, fixation, permeabilization and blocking, cells were incubated with commercial anti-NRP-1 (NBP2-67539, Novus) and anti-OBR (AF389, R&D Systems) antibodies at 10 μg/ml for 1 h at 37° C. As a negative control for the PLA, cells were incubated only with the commercial anti-NRP-1 antibody. .. The PLA positive signal (FarRed) was detected by the LSR Fortessa cytometer (BD Biosciences).

    Incubation:

    Article Title: Neuropilin-1-peptide based antibody, highlighting new epitope associated to glycosaminoglycan-modified neuropilin-1 and methods of use thereof
    Article Snippet: To analyze the a-NRP1_LEP antibody effect on the NRP-1/OBR complex formation, the MDA-MB-231 cells were cultured for two days in human serum (H4522, Sigma) and then treated for two additional days with the mouse a-NRP1_LEP antibody of the invention or its mIgG2b isotype control at 10, 20 or 40 μg/ml, or with the antibody of the prior art a-NRP1_VE and its hIgG1 isotype control at 20 μg/ml. .. After washes, fixation, permeabilization and blocking, cells were incubated with commercial anti-NRP-1 (NBP2-67539, Novus) and anti-OBR (AF389, R&D Systems) antibodies at 10 μg/ml for 1 h at 37° C. As a negative control for the PLA, cells were incubated only with the commercial anti-NRP-1 antibody. .. The PLA positive signal (FarRed) was detected by the LSR Fortessa cytometer (BD Biosciences).

    Negative Control:

    Article Title: Neuropilin-1-peptide based antibody, highlighting new epitope associated to glycosaminoglycan-modified neuropilin-1 and methods of use thereof
    Article Snippet: To analyze the a-NRP1_LEP antibody effect on the NRP-1/OBR complex formation, the MDA-MB-231 cells were cultured for two days in human serum (H4522, Sigma) and then treated for two additional days with the mouse a-NRP1_LEP antibody of the invention or its mIgG2b isotype control at 10, 20 or 40 μg/ml, or with the antibody of the prior art a-NRP1_VE and its hIgG1 isotype control at 20 μg/ml. .. After washes, fixation, permeabilization and blocking, cells were incubated with commercial anti-NRP-1 (NBP2-67539, Novus) and anti-OBR (AF389, R&D Systems) antibodies at 10 μg/ml for 1 h at 37° C. As a negative control for the PLA, cells were incubated only with the commercial anti-NRP-1 antibody. .. The PLA positive signal (FarRed) was detected by the LSR Fortessa cytometer (BD Biosciences).

    Proximity Ligation Assay:

    Article Title: Neuropilin-1-peptide based antibody, highlighting new epitope associated to glycosaminoglycan-modified neuropilin-1 and methods of use thereof
    Article Snippet: To analyze the a-NRP1_LEP antibody effect on the NRP-1/OBR complex formation, the MDA-MB-231 cells were cultured for two days in human serum (H4522, Sigma) and then treated for two additional days with the mouse a-NRP1_LEP antibody of the invention or its mIgG2b isotype control at 10, 20 or 40 μg/ml, or with the antibody of the prior art a-NRP1_VE and its hIgG1 isotype control at 20 μg/ml. .. After washes, fixation, permeabilization and blocking, cells were incubated with commercial anti-NRP-1 (NBP2-67539, Novus) and anti-OBR (AF389, R&D Systems) antibodies at 10 μg/ml for 1 h at 37° C. As a negative control for the PLA, cells were incubated only with the commercial anti-NRP-1 antibody. .. The PLA positive signal (FarRed) was detected by the LSR Fortessa cytometer (BD Biosciences).



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    Santa Cruz Biotechnology lepr obr
    IL-6 and <t>LEPR</t> immunostaining in control and preeclamptic placentas. Representative images of IL-6 and LEPR immunostaining in preeclamptic (PE) and gestational aged-matched control placentas. Hofbauer cells-specific HSCORE analysis demonstrated significantly higher IL-6 (n = 5/group) and LEPR expression in PE placentas (n = 8) vs. gestational aged-matched controls (n = 7) (83.0 ± 20.9 vs. 25.0 ± 4.5 and 63.5 ± 18.6 vs. 10.9 ± 1.0, respectively). Arrows indicate IL-6 and LEPR-expressing Hofbauer cells. Bars represent median values, * p < 0.05 vs. control (C) specimens analyzed by Mann–Whitney Rank Sum test. Scale bar = 30 µm.
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    Image Search Results


    Pearson’s correlation (R) between anthropometric and biochemical variables and the mRNA expression (a.u.) of genes in study tissues.

    Journal: International Journal of Molecular Sciences

    Article Title: Evidence That Peripheral Leptin Resistance in Omental Adipose Tissue and Liver Correlates with MASLD in Humans

    doi: 10.3390/ijms25126420

    Figure Lengend Snippet: Pearson’s correlation (R) between anthropometric and biochemical variables and the mRNA expression (a.u.) of genes in study tissues.

    Article Snippet: The tissue was incubated overnight with a 1:10 dilution of anti-ObR/ LEPR antibody (B:3) (sc-8391; Santa Cruz Biotechnology, Dallas, TX, USA), followed by anti-mouse peroxidase-labeled secondary antibody.

    Techniques: Expressing, Fat

    Pearson’s correlation (R) between the mRNA expression (a.u.) of genes in study tissues.

    Journal: International Journal of Molecular Sciences

    Article Title: Evidence That Peripheral Leptin Resistance in Omental Adipose Tissue and Liver Correlates with MASLD in Humans

    doi: 10.3390/ijms25126420

    Figure Lengend Snippet: Pearson’s correlation (R) between the mRNA expression (a.u.) of genes in study tissues.

    Article Snippet: The tissue was incubated overnight with a 1:10 dilution of anti-ObR/ LEPR antibody (B:3) (sc-8391; Santa Cruz Biotechnology, Dallas, TX, USA), followed by anti-mouse peroxidase-labeled secondary antibody.

    Techniques: Expressing

    IL-6 and LEPR immunostaining in control and preeclamptic placentas. Representative images of IL-6 and LEPR immunostaining in preeclamptic (PE) and gestational aged-matched control placentas. Hofbauer cells-specific HSCORE analysis demonstrated significantly higher IL-6 (n = 5/group) and LEPR expression in PE placentas (n = 8) vs. gestational aged-matched controls (n = 7) (83.0 ± 20.9 vs. 25.0 ± 4.5 and 63.5 ± 18.6 vs. 10.9 ± 1.0, respectively). Arrows indicate IL-6 and LEPR-expressing Hofbauer cells. Bars represent median values, * p < 0.05 vs. control (C) specimens analyzed by Mann–Whitney Rank Sum test. Scale bar = 30 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis

    doi: 10.3390/ijms25010135

    Figure Lengend Snippet: IL-6 and LEPR immunostaining in control and preeclamptic placentas. Representative images of IL-6 and LEPR immunostaining in preeclamptic (PE) and gestational aged-matched control placentas. Hofbauer cells-specific HSCORE analysis demonstrated significantly higher IL-6 (n = 5/group) and LEPR expression in PE placentas (n = 8) vs. gestational aged-matched controls (n = 7) (83.0 ± 20.9 vs. 25.0 ± 4.5 and 63.5 ± 18.6 vs. 10.9 ± 1.0, respectively). Arrows indicate IL-6 and LEPR-expressing Hofbauer cells. Bars represent median values, * p < 0.05 vs. control (C) specimens analyzed by Mann–Whitney Rank Sum test. Scale bar = 30 µm.

    Article Snippet: LepR (ObR) , Santa Cruz #sc-8391(B-3) , IHC 1:100.

    Techniques: Immunostaining, Control, Expressing, MANN-WHITNEY

    Antibodies used in this study. WB: Western blotting, IHC: Immunohistochemistry.

    Journal: International Journal of Molecular Sciences

    Article Title: Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis

    doi: 10.3390/ijms25010135

    Figure Lengend Snippet: Antibodies used in this study. WB: Western blotting, IHC: Immunohistochemistry.

    Article Snippet: LepR (ObR) , Santa Cruz #sc-8391(B-3) , IHC 1:100.

    Techniques: Western Blot, Immunohistochemistry